The effect of substituting Platinum HiFi PCR supermix with Kapa HiFi in the PGM library prep amplification step. A) The percentage of the P. falciparum genome covered at different read depths. The blue line shows the data obtained with the recommended Platinum enzyme and the green line with Kapa HiFi. The red line depicts ideal coverage behavior. B) The number of bases covered at different depths. C) Sequence representation vs. GC-content plots.
Quail et al. BMC Genomics 2012 13:341 doi:10.1186/1471-2164-13-341