Table 2

Validation of microarray results by real-time quantitative PCR
Gene expression fold change after ETEC infection in each group
(Inf./Non.)
CF4abvscontrol* CF4acvscontrol CF18acvscontrol
Genes Probe Name in Micro-array Micro-array Q-PCR# Genes Probe Name in Micro-array Micro-array Q-PCR Genes Probe Name in Micro-array Micro-array Q-PCR
IL8 A_72_P232367 5.03** 4.09**(G) 5.50*(A) MUC20 A_72_P440416 −1.33* −1.82**(G) −1.23(A) FUT1 A_72_P146581 1.13 1.00(G) 1.04(A)
SDC2 A_72_P098981 −2.57** −2.84**(G) −2.06(A) ANK3 A_72_P628816 −2.90** −4.40**(G) −2.99(A) CXCL2 A_72_P146411 2.31* 2.31*(G) 2.29*(A)
UPK2 A_72_P441773 4.27** 3.69**(G) 5.21*(A) IRG6 A_72_P443124 4.86** 4.67*(G) 6.75**(A) IRG6 A_72_P443124 2.12* 1.62(G) 1.64*(A)
MUC4 A_72_P140196 −1.46 −2.04(G) −1.28(A) LOC10015251 A_72_P527727 −1.25 −1.91(G) −1.42(A)
MUC13 A_72_P442057 −3.01** −5.66**(G) −3.46**(A)

* CF4ab, IPEC-J2 cells infected with F4ab ETEC; CF4ac, IPEC-J2 cells infected with F4ac ETEC ; CF18ac, IPEC-J2 cells infected with F18ac ETEC ; Control: the non-infected IPEC-J2 cells. # G: gene expression level related to GAPDH; A: gene expression level related to ACTB. The numbers here represent the fold change of gene expression after ETEC infection. The numbers equal to 1.00 means the expression level doesn’t change after ETEC infection. The numbers > 1.00 means the expression level is increased after ETEC infection while numbers < 1.00 indicate that the expression level is reduced after ETEC infection. The numbers with “*” are statistically significant (P < 0.05), and which with“**” are statistically very significant (P < 0.01).

Zhou et al.

Zhou et al. BMC Genomics 2012 13:330   doi:10.1186/1471-2164-13-330

Open Data