Table 1

Twenty-five common up-regulated genes ofB. pseudomalleiduring intracellular growth in host macrophages relative toin vitrogrowth
Gene Description Fold Change (in vivo/in vitro) at the indicated time (h)
1 2 4 6
BPSL0184 Putative rod shape-determining protein 23.83 15.31 12.69 12.06
BPSL0842 Benzoylformate decarboxylase 70.27 31.78 27.64 20.64
BPSL0886 Hypothetical protein 12.29 8.36 6.56 5.20
BPSL1067 Hypothetical protein 8.39 5.15 6.75 6.27
BPSL1771 Cobalamin biosynthesis protein CbiG 25.36 16.66 15.21 15.76
BPSL1817 Putative lipoprotein 30.07 14.11 12.67 11.33
BPSL1902 Hypothetical protein 37.91 18.81 12.46 8.39
BPSL2759 Putative short-chain dehydrogenase 16.54 8.31 5.93 5.61
BPSL2945 Allantoicase 27.90 9.57 8.83 7.41
BPSL3354 Putative cytochrome 18.66 11.70 8.64 7.62
BPSS0140 Sugar ABC transport system, lipoprotein 7.80 16.62 13.56 13.94
BPSS0142 * Sugar ABC transport system, ATP-binding protein 4.62 10.04 11.34 18.79
BPSS0143 ROK family transcriptional regulator 4.78 9.97 11.80 18.64
BPSS0404 Methylamine utilization protein 49.46 36.31 30.14 23.42
BPSS0433 Hypothetical protein 11.87 5.84 7.70 10.59
BPSS0529 Lipoprotein 26.49 19.04 14.10 10.68
BPSS1279 Threonine dehydratase 17.47 11.84 10.80 11.03
BPSS1498 * tssD, type VI secretion system Hcp protein 7.38 93.61 130.11 181.92
BPSS1499 tssE, type VI secretion system lysozyme 4.72 58.87 99.06 160.21
BPSS1505 tagB, type VI secretion system hypothetical protein 6.49 14.01 18.40 27.97
BPSS1508 tssJ, type VI secretion system lipoprotein 5.04 6.27 8.17 9.88
BPSS1728 * Secretion/activator protein 3.00 23.60 29.33 24.54
BPSS1892 catA, catechol 1,2-dioxygenase 7.76 19.46 16.64 16.53
BPSS1893 catC, muconolactone delta-isomerase 10.57 9.30 7.33 6.30
BPSS2276 LysR family regulatory protein 59.21 41.83 26.33 19.45

Note: * Genes selected for real-time qPCR analysis.

Chieng et al.

Chieng et al. BMC Genomics 2012 13:328   doi:10.1186/1471-2164-13-328

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