Testing of sequence-specific PCR-based molecular markers “AnSeq1”, “AnSeq2” and “AnSeq5” on 26 F8recombinant inbred lines from a cross of Unicrop (susceptible to anthracnose disease) x Tanjil (resistant) of lupin (Lupinus angustifolius L.). “AnSeq1R”, “AnSeq2R” and“AnSeq5R” indicate the marker allele bands linked to disease resistance gene Lanr1. “AnSeq1S”, “AnSeq2S” and “AnSeq5S” indicate the marker allele bands associated with disease susceptibility allele. Disease phenotypes of the RILs are presented as “S” (susceptible) or “R” (resistant). A marker band with a vertical arrow indicates that a genetic recombination occurred between the R gene and marker locus on the chromosome in that particular plant for that particular marker. All other un-marked marker bands showed the correct match between the marker genotypes and the disease resistance phenotypes on these testing plants.
Yang et al. BMC Genomics 2012 13:318 doi:10.1186/1471-2164-13-318