A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation
1 INRA, UMR1083 SPO, 2 place Viala, F-34060, Montpellier, France
2 INRA, MIG, F-78350, Jouy-en-Josas, France
BMC Genomics 2012, 13:317 doi:10.1186/1471-2164-13-317Published: 17 July 2012
Additional file 1 :
Differentially expressed genes involved in the response to increases in NADPH and NADH oxidation. The differentially expressed genes involved in the response to increases in the oxidation of NADPH (100, 200 and 300 mM acetoin) and NADH (200 mM acetoin) are shown.
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Additional file 2 :
13 C-flux analysis:S. cerevisiae metabolic network, flux distribution in response to acetoin additions (0, 100, 200, 300 mM) and measured SFL and experimental data. (A) Reactions of the metabolic network used for 13 C-flux analysis. (B) Fluxes estimated by 13 C-flux analysis. (C) Measured SFL. (D) Specific growth rate, glucose uptake rates and yield coefficients used in 13 C flux.
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