Additonal file 8 .
Figure S6. Distribution of Class I and II elements identified in the Vd.Ls17 genome in other phytopathogenicVerticilliumspp. isolates. A) Southern hybridization analysis of the indicated Vd and Va fungal isolates for the presence of retrotransposons. Genomic DNA was digested with EcoRI (top panel), BglII (middle panel), or SacI (bottom panel), and blots hybridized with DIG-labelled probes corresponding to VdLTRE1, VdLTRE2/3/4, or VdLTRE5, respectively. Sizes of DIG-labelled molecular weight markers (Kb) are indicated to the right of the images; B) PCR amplifications of retrotransposons in the indicated Vd and Va isolates, using VdLTRE1, VdLTRE2 and VdLTRE5 primer pairs. Amplification of actin was used as control (not shown); FSDW, no-template reaction; C) PCR amplification of DAHLIAE, VdHAT and VdMULE elements was obtained by using as a template genomic DNA (20 ng) of the indicated Vd and Va isolates; D) To verify specificity of the bands of different size obtained from amplification of DAHLIAE 1d, PCR products were blotted onto nylon membranes and hybridized with DIG-labelled specific probes. Details of the isolates used in the survey are provided in Table 3. Sequences of the primers used in the survey are listed in Supplemental Table 3.
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Amyotte et al. BMC Genomics 2012 13:314 doi:10.1186/1471-2164-13-314