Additonal file 6 .
Figure S4. Analysis of the expression of VdLs. 17 Class II transposases in response to heat stress. The transcription of the indicated Class II transposases was analyzed by RT-PCR experiments using cDNA synthetized from total RNA extracted from VdLs.17 grown at 25°C or exposed to heat stress (42°C for 45 minutes). The primers used to amplify the transposase ORFs were designed on the basis of the sequence of the corresponding ESTs publicly available at the Verticillium Group Database (http://www.broadinstitute.org/annotation/genome/verticillium_dahliae/MultiHome.html webcite) (Primer sequences are listed in Supplemental Table 3).
Format: DOCX Size: 61KB Download file
Amyotte et al. BMC Genomics 2012 13:314 doi:10.1186/1471-2164-13-314