Validation of differentially expressed mRNA. When comparing dwarf and normal chickens, the expression levels of let-7b were significantly up-regulated. The mRNA expression levels of GHR and SOCS3 were significantly up-regulated, but those of IRS1, LEPR and JAK2 are significantly down-regulated. All mRNA expression levels of MYOD1, MyoG and Myf5 regulating the growth of skeletal muscle were significantly up-regulated in normal chickens, and significantly down-regulated in dwarf chickens. The mRNA expression levels of IGF1 and IGF2BP3, associated with the insulin signaling pathway, were significantly down-regulated in dwarf chickens.
Lin et al. BMC Genomics 2012 13:306 doi:10.1186/1471-2164-13-306