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Open Access Research article

Transcriptional profiling by cDNA-AFLP analysis showed differential transcript abundance in response to water stress in Populus hopeiensis

Yuepeng Song12, Zeliang Wang12, Wenhao Bo12, Yuanyuan Ren12, Zhiyi Zhang1 and Deqiang Zhang12*

Author Affiliations

1 National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China

2 Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China

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BMC Genomics 2012, 13:286  doi:10.1186/1471-2164-13-286

Published: 29 June 2012

Additional files

Additional file 1 :

Figure S1. Physiological response to water-deficit stress inP.hopeiensi(P. tomentosaas a control). A, Changes in soluble sugar content in response to water-deficit stressed. B, Changes in soluble protein content in response to water-deficit stress. C, Changes in malondialdehyde (MDA) content in response to water-deficit stress stage, four water-deficit stress time points.

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Additional file 2 :

Table S1. cDNA-AFLP primer information.

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Additional file 3 :

Figure S2. GO categorization of differentially expressed poplar genes during the 20% fresh weight loss.

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Additional file 4 :

Figure S3. GO categorization of differentially expressed poplar genes during the 30% fresh weight loss.

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Additional file 5 :

Figure S4. GO categorization of differentially expressed poplar genes during the 50% fresh weight loss stage.

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Additional file 6 :

Table S2. Transcript-derived fragments (TDFs) from P. hopeiensis showing drought-responsive expression, with homologies to genes in TAIR.

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Additional file 7 :

Figure S5. Stable expression of poplarACTINII-like(accession number: EF145577) andUBIQUITINgenes during the water stress used as the internal control.

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Additional file 8 :

Figure S6. TDF125 and 189 expression pattern response to stress. A–E, representing cold, heat, H2O, NaCl, and ABA stress respectively.1-7, 0, 0.5, 1, 2, 5, 10, and 24 h time points after stress initiation.

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Additional file 9 :

Figure S7. Expression patterns of genes coding for unknown proteins.

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Additional file 10 :

Table S3. OD values of RNA samples.

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Additional file 11 :

Figure S8. Gel photos of RNA quality. Stage1-4, the four water treatment time points. S1-3, triplicate for biological repetitions.

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Additional file 12 :

Table S4. RT-PCR primer information.

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