Additional file 3.

In vitro T-cell stimulating capacity of natural variants of celiac disease epitope, DQ2-γ-I. Stimulation of a T-cell clone specific for CD epitope DQ2-γ-I, with natural occurring variants of DQ2-γ-I (9-mer epitope core PQQSFPQQQ and residues in the positions −1 to −4 and +1 to +4). Glutamine residues that are a primary targets for the enzyme tissue transglutaminase are underlined (

    Q
) in QxP target sites whereas moderate target sites are depicted in italics (Q) [13,14]. SI = Stimulation Index = cpm of the stimulated culture/cpm unstimulated culture (with APC only); ++ = SI ≥ 50, + = 20 ≤ SI <50, ± =10 ≤ SI <20, - = SI <10. The stimulation is mediated by HLA-DQ2 carrying antigen presenting cells (APC). Peptide no. corresponds to the peptide numbering in Table 5.

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Salentijn et al. BMC Genomics 2012 13:277   doi:10.1186/1471-2164-13-277