Concordance in expression profiles in heart tissues. Pair-wise comparisons of all cardiac expressed genes (~8,400 based on the GNF anatomical system data: heart), expressed as the fold change between time-points. To establish a baseline level of expected concordance for technical replicates, we compared the expression profile of two HeLa controls, and observed 94.9% of genes with ≤1.25 fold change (red line). Comparing the baseline explant and autopsy to later timepoints, we saw no difference from technical replicates (95.9% and 93.4% ≤1.25 fold change, respectively) (purple and brown lines). However, comparing the autopsy to the explant, we saw a measurable difference (86.8% ≤1.25 fold change) (black line), and comparing either heart sample to the HeLa controls demonstrated a striking difference (48% ≤1.25 fold change) (green and blue lines).
Gupta et al. BMC Genomics 2012 13:26 doi:10.1186/1471-2164-13-26