Table 1

Breed and genotype of individuals sequenced for the presence ofFGF20c.535A > T
Breed Genotype No. of individuals c.496 (rs14481412) c.535 c.552 (rs14481413)
UC Davis (low line) sc/sc 10 CC TT AA
UC Davis (high line) sc/sc 10 CC TT AA
Storrs, Connecticut (low line) sc/sc 2 CC TT AA
Israeli experimental line sc/sc 16 CC TT AA
Israeli experimental line sc/+ 3 CC AT AA
Israeli experimental line sc/+ 6 CC AT AG
New Hampshire (IFAG, Germany) WT 6 CC AA AA
New Hampshire (IFAG, Germany) WT 4 CT AA AG
New Hampshire, UK WT 2 TT AA GG
New Hampshire, UK WT 1 CT AA AG
Black Leghorn WT 1 CC AA AA
Araucana WT 1 CC AA AA
Old English Pheasant Fowl WT 1 CC AA AA
Langshan WT 1 CT AA AG
Silver Appenzeller WT 1 CC AA AA
Roslin Institute experimental WT 2 CC AA AA
Roslin Institute experimental WT 1 CT AA AG
ISA Brown WT 2 CC AA AA
Outbred, Mexico WT 7 CC AA AA
Outbred, Mexico WT 2 TT AA GG
Outbred, Mexico WT 1 CT AA AG
Cream Legbar WT 1 CC AA AA
Dorking WT 1 CC AA AA
Campine WT 1 CC AA AA
Naked Neck, Wernlas collection WT 1 TT AA GG
Hungarian AVIANDIV Naked Neck WT 1 TT AA GG
Naked Neck, English WT 1 CC AA AA
Scots Grey WT 1 TT AA GG

Results from sequencing FGF20 exon 3 in 38 sc/sc individuals, 9 sc/+ individuals and 40 WT birds. The FGF20 c.535A > T mutation was found in all of the sc/sc and none of the WT samples sequenced. The genotype of the two flanking SNPs (rs14481412 and rs14481413) revealed that 25/40 WT individuals were homozygous for the same haplotype as scaleless, indicating that the FGF20 c.535A > T mutation does not lie within a rare haplotype.

Wells et al.

Wells et al. BMC Genomics 2012 13:257   doi:10.1186/1471-2164-13-257

Open Data