Table 2

Relative abundance of Unitrans annotated as having a predicted function in defense response in six libraries representing different elm leaf treatments
Gene description based on homology # of Unitrans # of ESTs Best database match E-value Treatment (pptt)
EF EF + F E F MeJA C
PR Proteins
 Class I chitinase 12 2111 Brassica napus 8e-58 161 70 28 13 133 33
 Disease resistance response protein 5 192 Arabidopsis thaliana 4e-25 - 1 - - 1 -
 Glucan endo-1,3-beta-glucosidase 7 190 Prunus persica 2e-115 10 2 19 - 7 -
 Cysteine proteinase inhibitor 3 189 Vigna unguiculata 1e-26 5 5 - - 6 5
 MLP-like protein 3 86 Arabidopsis thaliana 3e-42 86 10 2 3 8 3
 Pathogenesis-related protein 3 34 Medicago truncatula 5e-22 - 0.3 - 2 - -
 MLO-like protein 2 4 Arabidopsis thaliana 4e-32 - 0.1 - - - -
Phytohormone signaling
 Jasmonate ZIM-domain protein 3 1 111 Arabidopsis thaliana 3e-24 21 3 - 2 10 -
 Ethylene-responsive transcription factor 3 97 Arabidopsis thaliana 1e-41 - 3 5 2 4 9
 Auxin signaling F-box 2 2 33 Arabidopsis thaliana 3e-79 21 1 5 2 1 -
 ABC transporter G family member 40- 1 10 Arabidopsis thaliana 1e-62 - 0.1 - 0.1 1 -
 Regulatory protein NPR1 1 7 Arabidopsis thaliana 1e-25 9 - 0.2 0.3 - -
 Coronatine-insensitive protein 1 1 8 Arabidopsis thaliana 9e-54 - 0.1 - - - -
 Probable WRKY transcription factor 33 1 4 Arabidopsis thaliana 7e-28 16 - - - - -
 Ethylene-insensitive protein 2 1 2 Arabidopsis thaliana 9e-23 - 0.1 - - - -
Jasmonic acid synthesis
 Allene oxide synthase 4 391 Linum usitatissimum 3e-39 10 12 9 13 30 5
 Peroxisomal acyl-coenzyme A oxidase 1 2 8 Arabidopsis thaliana 6e-35 5 0.1 - - - -
Innate immunity
 Pre-mRNA-splicing factor SPF27 1 13 Arabidopsis thaliana 3e-52 - 0.5 - - - -
 Pre-mRNA-processing factor 19 4 11 Arabidopsis thaliana 2e-29 - 0.3 - - 1 -
 Cell division cycle 5-like protein 2 11 Arabidopsis thaliana 5e-50 - 0.3 - - - -
 Protein pleiotropic regulatory locus 1 2 7 Arabidopsis thaliana 5e-75 - 0.3 - - - -
 Serine / threonine-protein kinase PBS1 1 2 Arabidopsis thaliana 7e-42 - 0.1 - - - -
Regulatory role in defense response
 Patatin-like protein 1 557 Solanum tuberosum 7e-83 47 13 9 15 48 -
 Heat shock protein 81 4 309 Arabidopsis thaliana 0 - 5 - 2 3 -
 Ankyrin repeat domain-containing protein 2 4 136 Arabidopsis thaliana 5e-59 - 3 9 19 4 -
 (+)-neomenthol dehydrogenase 3 29 Arabidopsis thaliana 2e-23 - 1 - 2 1 -
 Cyclic nucleotide-gated ion channel 3 15 Arabidopsis thaliana 2e-45 10 0.3 - 0.1 - -
 Two pore calcium channel protein 1 2 5 Nicotiana tabacum 6e-31 - 0.2 - - - -
Cell wall metabolism
 Cellulose synthase A catalytic subunit 3 3 17 Arabidopsis thaliana 5e-63 - 1 - - - -

Libraries: C (untreated control), E (artificial scratching & eggs transferred), EF (egg laying & feeding), F (feeding), MeJA (methyl jasmonate), mixed library EF + F. Relative Unitrans abundance calculated on library counts by parts per ten thousand (pptt) based on the annotation to Plant Swiss Prot (BLASTx, E-value ≤ 1e-20). Annotated transcripts filtered depending on their predicted function to the category “GO:0006952 defense response” of the Gene Ontology term. Bold = increased relative Unitrans abundance in egg-induced plants.

Büchel et al.

Büchel et al. BMC Genomics 2012 13:242   doi:10.1186/1471-2164-13-242

Open Data