Table 1

Sequencing output of elm libraries
cDNA Librariesa ESTs Unitransb ≥ 2 ESTs Library specific Unitransc Singletons (%)d
Untreated control (C) 2132 836 31 174 (17)
Egg & feeding (EF) 1921 826 50 211 (20)
Feeding (F) 4725 1453 65 326 (18)
Methyl jasmonate (MeJA) 7080 2272 153 679 (23)
Transferred eggs (E) 2133 811 40 188 (19)
Mixe          EF + F 169672 15083 2844 11560 (43)
       C + MeJA + E 71239 9141 860 8043 (47)
   C + EF + F + MeJA + E 98210 12402 2249 9755 (44)
Tag unidentifiable 4084 597 200 397 (40)
Total 361196 21490 31333 (59)

a Libraries of differently treated elms: C = Control (untreated Ulmus minor leaves); EF = Egg laying & feeding (=leaves with elm leaf beetle eggs and feeding damage by female beetles [natural situation]; F = Feeding (=leaves with feeding damage by male beetles); Methyl jasmonate (= leaves treated with 50 μmol MeJA), E = Transferred eggs (= leaves that had been scratched and had eggs artificially placed on them ); b Number of Unitrans (unique transcripts) ≥2 ESTs that contain at least one EST of one of the libraries; c Number of Unitrans ≥2 ESTs that contain ESTs of only the particular library; d Percent singletons in relation to all unique transcripts (Unitrans ≥2 ESTs + singletons); e New sequencing runs of combined libraries.

Büchel et al.

Büchel et al. BMC Genomics 2012 13:242   doi:10.1186/1471-2164-13-242

Open Data