|Sequencing output of elm libraries|
|cDNA Librariesa||ESTs||Unitransb ≥ 2 ESTs||Library specific Unitransc||Singletons (%)d|
|Untreated control (C)||2132||836||31||174 (17)|
|Egg & feeding (EF)||1921||826||50||211 (20)|
|Feeding (F)||4725||1453||65||326 (18)|
|Methyl jasmonate (MeJA)||7080||2272||153||679 (23)|
|Transferred eggs (E)||2133||811||40||188 (19)|
|Mixe EF + F||169672||15083||2844||11560 (43)|
|C + MeJA + E||71239||9141||860||8043 (47)|
|C + EF + F + MeJA + E||98210||12402||2249||9755 (44)|
|Tag unidentifiable||4084||597||200||397 (40)|
a Libraries of differently treated elms: C = Control (untreated Ulmus minor leaves); EF = Egg laying & feeding (=leaves with elm leaf beetle eggs and feeding damage by female beetles [natural situation]; F = Feeding (=leaves with feeding damage by male beetles); Methyl jasmonate (= leaves treated with 50 μmol MeJA), E = Transferred eggs (= leaves that had been scratched and had eggs artificially placed on them ); b Number of Unitrans (unique transcripts) ≥2 ESTs that contain at least one EST of one of the libraries; c Number of Unitrans ≥2 ESTs that contain ESTs of only the particular library; d Percent singletons in relation to all unique transcripts (Unitrans ≥2 ESTs + singletons); e New sequencing runs of combined libraries.
Büchel et al.
Büchel et al. BMC Genomics 2012 13:242 doi:10.1186/1471-2164-13-242