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Open Access Research article

Leaf morphology in Cowpea [Vigna unguiculata (L.) Walp]: QTL analysis, physical mapping and identifying a candidate gene using synteny with model legume species

Marti Pottorff1, Jeffrey D Ehlers12, Christian Fatokun3, Philip A Roberts4* and Timothy J Close1*

Author Affiliations

1 Department of Botany & Plant Sciences, University of California Riverside, Riverside, CA, USA

2 Bill & Melinda Gates Foundation, Seattle, WA, USA

3 International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria

4 Department of Nematology, University of California Riverside, Riverside, CA, USA

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BMC Genomics 2012, 13:234  doi:10.1186/1471-2164-13-234

Published: 12 June 2012

Additional files

Additional file 1:

Cowpea accessions with a hastate or sub-globose leaf phenotype.

Format: DOCX Size: 12KB Download file

Open Data

Additional file 2:

SNP marker 1_0349 sequence. cDNA sequence of P12 assembly unigene 8605 which is housed in Harvest:Cowpea database (http://harvest.ucr.edu webcite). The SNP (thymine/cytosine) is located at position 2122, parenthesized, underlined and in bold.

Format: DOCX Size: 11KB Download file

Open Data

Additional file 3:

Synteny of the Hls locus with A. thaliana. Synteny was examined for the Hls locus between cowpea and A. thaliana using EST-derived SNP markers previously BLASTed and aligned to the sequenced genome. The Hls locus on the cowpea consensus genetic map, linkage group 4 (25.57 cM – 35.96 cM position), showed very low synteny with the Arabidopsis genome. The syntenic map was drawn using HarvEST:Cowpea database (http://harvest.ucr.edu webcite) using a cut off e-score value of -10 and a minimum number of 10 lines drawn per linkage group.

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Open Data

Additional file 4:

Summary of significant markers in the Hls locus.

Format: DOCX Size: 12KB Download file

Open Data