Table 1

Relative changes in protein expression on overexpression of Yap1p.1
Protein Description ORF Normalized averaged spot quantity Y/C p-value YBS
Control Yap1+
Avol SD Avol SD
Protein involved in carbon metabolism pathways
Glycolysis
Fba1p# Fructose-bisphosphate aldolase YKL060C 3134 89 5986 1035 1.91 0.009 +
Pgi1p# Glucose-6-phosphate isomerase YBR196C 540 120 950 205 1.76 0.040 -
Hxk2p Hexokinase-2 YGL253W 167 10 374 92 2.24 0.018 -
Tdh1p Glyceraldehyde-3-phosphate dehydrogenase 1 YJL052W 709 148 1390 256 1.96 0.018 +
Tdh2p Glyceraldehyde-3-phosphate dehydrogenase 2 YJR009C 1769 118 2516 539 1.42 0.079 -
Tdh3p Glyceraldehyde-3-phosphate dehydrogenase 3 YGR192C 6899 401 9620 195 1.39 <0.001 +
Pgk1p# Phosphoglycerate kinase YCR012W 3970 506 5204 931 1.31 0.045 +
Gpm1p Phosphoglycerate mutase 1 YKL152C 2960 508 4727 284 1.60 0.006 +
Eno2p# Enolase 2 YHR174W 11810 323 15053 2147 1.27 0.041 +
Cdc19p# Pyruvate kinase 1 YAL038W 1460 44 3155 568 2.16 0.007 +
Pyruvate branchpoint
Pdc1p# Pyruvate decarboxylase isozyme 1 YLR044C 7914 380 7443 1520 1.55 0.043 -
Adh1p Alcohol dehydrogenase 1 YOL086C 4200 665 5158 743 1.31 0.041 +
Ald6p Aldehyde dehydrogenase YPL061W 161 33 329 40 2.04 0.005 +
Dld3p D-lactate dehydrogenase 3 YEL071W 290 20 501 79 1.73 0.011 -
Pentose phosphate pathway
Tkl1p# Transketolase 1 YPR074C 284 10 619 165 2.18 0.025 +
Gnd1p 6-phosphogluconate dehydrogenase 1 YHR183W 633 124 876 116 1.37 0.075 -
Protein involved in pathways other than carbon metabolism
Amino-acid and nucleotide metabolism
Sah1p Adenosylhomocysteinase YER043C 631 143 1310 304 2.08 0.025 -
Shm2p Serine hydroxymethyltransferase YLR058C 486 26 944 176 1.94 0.011 -
Aro9p Aromatic amino acid aminotransferase 2 YHR137W 116 19 164 13 1.41 0.022 -
Lys9p Saccharopine dehydrogenase YNR050C 147 13 364 123 2.48 0.039 +
Protein biosynthesis
Eft1p# Elongation factor 2 YOR133W 136 58 456 174 3.35 0.039 +
Yef3p Elongation factor 3A YLR249W 221 37 378 41 1.71 0.008 +
Gus1p Glutamyl-tRNA synthetase YGL245W 100 17 254 29 2.54 0.001 +
Tef4p Elongation factor 1-gamma 2 YKL081W 846 20 1059 11 1.25 0.004 +
Sgt2p Small glutamine-rich tetratricopeptide repeat-containing protein 2 YOR007C 588 67 855 101 1.45 0.019 +
Rps7ap 40 S ribosomal protein S7-A YOR096W 1377 459 2742 136 1.99 0.008 +
Grs1p Glycyl-tRNA synthetase 1 YBR121C 254 37 361 50 1.42 0.042 +
Kar2p 78 kDa glucose-regulated protein YJL034W 97 17 303 100 3.13 0.024 -
Pdi1p# Protein disulfide-isomerase YCL043C 85 24 354 88 4.17 0.007 -
Rpl5p 60 S ribosomal protein L5 YPL131W 364 137 1298 329 3.56 0.066 -
Rpp0p 60 S acidic ribosomal protein P0 YLR340W 752 88 1153 161 1.53 0.019 -
Rps3p 40 S ribosomal protein S3 YNL178W 342 25 801 214 2.34 0.095 +
Pab1p# Polyadenylate-binding protein YER165W 301 52 484 63 1.61 0.018 +
Heat-shock and chaperone proteins
Ssa1p Heat shock protein YAL005C 282 83 792 247 2.81 0.027 +
Ssa2p# Heat shock protein YLL024C 1279 211 2021 405 1.58 0.048 -
Ssb1p# Heat shock protein YDL229W 258 64 558 141 2.16 0.028 +
Ssb2p Heat shock protein YNL209W 295 72 578 130 1.96 0.031 +
Hsp82p ATP-dependent molecular chaperone YPL240C 77 17 253 55 3.30 0.006 +
Hsc82p# ATP-dependent molecular chaperone YMR186W 209 44 487 161 2.32 0.045 -
Sse1p Heat shock protein homolog YPL106C 73 16 194 68 2.66 0.041 +
Antioxidants
Tsa1p Peroxiredoxin TSA1 YML028W 656 99 909 90 1.39 0.031 +
Respiration
Oye2p# NADPH dehydrogenase 2 YHR179W 2129 174 6677 1357 3.14 0.005 +
Atp2p ATP synthase subunit beta YJR121W 158 13 313 35 1.98 0.002 +
Cell cycle and growth regulation
Bfr1p Nuclear segregation protein YOR198C 228 79 558 227 2.53 0.066 +
Stm1p Suppressor protein YLR150W 2101 245 3565 444 1.70 0.007 +
Cdc48p# Cell division control protein 48 YDL126C 121 8 319 110 2.65 0.036 +
Vma1p V-type proton ATPase subunit A YDL185W 220 49 318 26 1.44 0.039 -
Vma2p V-type proton ATPase subunit B YBR127C 233 99 412 85 1.77 0.076 -
Sis1p Protein SIS1 YNL007C 314 186 702 79 2.24 0.029 +
Rnr4p Ribonucleoside-diphosphate reductase small chain 2 YGR180C 623 19 1293 317 2.08 0.022 -
Bgl2p Endo-beta-1,3-glucanase YGR282C 580 45 3453 328 5.95 0.007 +
Bmh1p Protein BMH1 YER177W 1119 141 1564 30 1.40 0.049 +
Srp1p Importin subunit alpha YNL189W 185 24 354 88 1.91 0.006 +
Others
Ola1p Uncharacterized GTP-binding protein YBR025C 281 20 449 79 1.59 0.024 +
Ynn4p Uncharacterized protein YNL134C YNL134C 438 176 1171 354 2.67 0.033 +

1The normalized volume of spots from three replicate 2-D gels was averaged and the standard deviation was calculated for each yeast transformant. A Student’s t-test was performed to determine if the relative change was statistically significant. If proteins were identified and quantified in more than one spot on the 2-D gels, the total spot volume was calculated (indicated with “#”). YBS, nucleotide sequence with (+) or without (−) predicted Yap1p-binding site; Avol, averaged value from three spot volumes; Y/C, the relative change (Yap1p-overexpression versus control).

Jun et al.

Jun et al. BMC Genomics 2012 13:230   doi:10.1186/1471-2164-13-230

Open Data