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Open Access Research article

Widespread uncoupling between transcriptome and translatome variations after a stimulus in mammalian cells

Toma Tebaldi12, Angela Re1, Gabriella Viero13, Ilaria Pegoretti1, Andrea Passerini2, Enrico Blanzieri2 and Alessandro Quattrone12*

Author Affiliations

1 Laboratory of Translational Genomics, Centre for Integrative Biology (CIBIO), University of Trento, 38123, Trento, Italy

2 University of Trento, Department of Information Engineering and Computer Science (DISI), 38123, Trento, Italy

3 National Research Council, Institute of Biophysics & Bruno Kessler Foundation, 38123, Trento, Italy

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BMC Genomics 2012, 13:220  doi:10.1186/1471-2164-13-220

Published: 6 June 2012

Abstract

Background

The classical view on eukaryotic gene expression proposes the scheme of a forward flow for which fluctuations in mRNA levels upon a stimulus contribute to determine variations in mRNA availability for translation. Here we address this issue by simultaneously profiling with microarrays the total mRNAs (the transcriptome) and the polysome-associated mRNAs (the translatome) after EGF treatment of human cells, and extending the analysis to other 19 different transcriptome/translatome comparisons in mammalian cells following different stimuli or undergoing cell programs.

Results

Triggering of the EGF pathway results in an early induction of transcriptome and translatome changes, but 90% of the significant variation is limited to the translatome and the degree of concordant changes is less than 5%. The survey of other 19 different transcriptome/translatome comparisons shows that extensive uncoupling is a general rule, in terms of both RNA movements and inferred cell activities, with a strong tendency of translation-related genes to be controlled purely at the translational level. By different statistical approaches, we finally provide evidence of the lack of dependence between changes at the transcriptome and translatome levels.

Conclusions

We propose a model of diffused independency between variation in transcript abundances and variation in their engagement on polysomes, which implies the existence of specific mechanisms to couple these two ways of regulating gene expression.

Keywords:
Polysomal; Profiling; Transcriptome; Translational; Control; Translatome