Table 2

Essential genes in E. coli that are not as strongly selected in Typhimurium or Typhi*
S. Typhimurium 14028 gene symbol Gene name Gene description Best hit in S. Typhimurium LT2 Best hit in S. Typhi Ty2a Best hit in ECOa STM1 transposons STM1 reads STY1 transposons STY1 reads STY2 transposonsb STY2 readsb STY2 transposonsc STY2 readsc
Percentile rank
STM14_0106 folA Dihydrofolate reductase STM0087 t0090 b0048 4 3 8 8 19 19 46 60
STM14_0217 folK 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase STM0183 t0191 b0142 12 22 18 16 25 56 16 21
STM14_2754 yejM Putative hydrolase STM2228 t0626 b2188 14 16 30 25 13 19 17 20
STM14_4041d degS Serine endoprotease STM3349 t3265 b3235 50 34 14 14 6 7 4 4
STM14_4193 trpS Tryptophanyl-tRNA synthetase STM3481 t4024 b3384 18 16 14 14 78 64 12 12
STM14_4208 yrfF Intracellular growth attenuator protein STM3495 t4011 b3398 1 4 19 19 70 42 12 27
STM14_4460 gpsA NAD(P)H-dependent glycerol-3-phosphate dehydrogenase STM3700 t3819 b3608 10 10 14 17 45 46 8 11
STM14_4921 ftsN E cell division protein STM4093 t3525 b3933 42 38 14 15 20 27 15 15
STM14_4971.J murB UDP-N- acetylenolpyruvoylglucosamine reductase STM4136 t3489 b3972 42 48 26 22 8 24 13 21

* Details for all genes can be found in Additional file 5: Table S2. Genes were ranked by the number of transposon insertions and number of independent reads per gene. Ranks above the 15th percentile are shown in bold.

a Best hits are listed only if gene is syntenic with S. Typhimurium strain 14028 and has at least 95% sequence identity.

b Growth on L-agar with “aro mix” (L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid) [3].

c Growth after six passages in LB broth [3].

d Considered essential in STY2 [3].

Canals et al.

Canals et al. BMC Genomics 2012 13:212   doi:10.1186/1471-2164-13-212

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