Open Access Research article

High-throughput comparison of gene fitness among related bacteria

Rocio Canals1, Xiao-Qin Xia2, Catrina Fronick3, Sandra W Clifton3, Brian MM Ahmer4, Helene L Andrews-Polymenis5, Steffen Porwollik6 and Michael McClelland16*

Author Affiliations

1 University of California, Irvine, CA, USA

2 Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China

3 Washington University, Saint Louis, MO, USA

4 Ohio State University, Columbus, OH, USA

5 Texas A&M University System Health Science Center, College Station, TX, USA

6 Vaccine Research Institute of San Diego, San Diego, CA, USA

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BMC Genomics 2012, 13:212  doi:10.1186/1471-2164-13-212

Published: 30 May 2012

Additional files

Additional file 1:

Table S1. Transposon libraries (EZ-Tn5 < T7/Kan-2>) assayed.

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Additional file 2:

Supplemental Methods. Detailed explanation of protocols.

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Additional file 3:

Figure S1. Diagram of sequencing protocol.

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Additional file 4:

Figure S2. Transposon insertion frequency across the S. Typhimurium 14028 s genome.

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Additional file 5:

Table S2. Essentiality surveys and transposon data.

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Additional file 6:

Table S3. STY1 complete transposon data.

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