PCE dehalogenase (Pce) genomic island on theG. lovleyistrain SZ chromosome (CP001089; Glov_2866-Glov_2875) (Panel A) compared to the transposon-encodedpce-gene cluster fromDesulfitobacterium hafniensestrain TCE1 (AJ439608) (Panel B). The transposase-associated pce-gene clusters in the genomes of D. hafniense strain Y51 (AP008230) and Dehalobacter restrictus PER-K23 (AJ439607) share 99% nucleotide identity with the strain TCE1 pce-gene cluster. Inferred gene functions are abbreviated as follows: pceA - putative tetrachloroethene (PCE) dehalogenase, pceB - membrane anchor protein subunit, pceT - peptidylprolyl-cis-trans-isomerase, pceC - FMN-binding and polyferredoxin domain protein, int - integrase, ist - ATPase, tr‒transposase, and ald‒aldehyde dehydrogenase. The pceA-pceB ORFs are duplicated within the strain SZ genomic island, and associated with transposase genes, but no inverted repeats were detected. In contrast, the RDase genes in strain TCE1 are associated with both transposases and inverted repeats, and the overall structure of the element in TCE1 resembles a composite transposon.
Wagner et al. BMC Genomics 2012 13:200 doi:10.1186/1471-2164-13-200