Open Access Highly Accessed Methodology article

Sequence based polymorphic (SBP) marker technology for targeted genomic regions: its application in generating a molecular map of the Arabidopsis thaliana genome

Binod B Sahu1, Rishi Sumit12, Subodh K Srivastava1 and Madan K Bhattacharyya12*

Author Affiliations

1 Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA

2 Molecular, Cellular and Developmental Biology Interdepartmental Graduate Program, Iowa State University, Ames, Iowa 5001, USA

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BMC Genomics 2012, 13:20  doi:10.1186/1471-2164-13-20

Published: 13 January 2012

Additional files

Additional file 1:

Arabidopsis molecular genome map generated based on SSLP and CAPS markers that are polymorphic between Col-0 and Nd-0 ecotypes. CAPS markers shown with asterisks. The map was drawn using the chromosome map tool available at TAIR.

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Additional file 2:

Phenotypes of the SSLP and CAPS markers polymorphic between Col-0 and Nd-0 ecotypes. C, Col-0; N, Nd-0.

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Additional file 3:

Two 75 bp Nd-0 Solexa reads most likely originated from a single DNA molecule. The two reads showed similarity in their identity to a specific Col-0 sequence. Most likely the two sequence reads were obtained from sequencing of two molecules generated through PCR of a single DNA molecule.

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Additional file 4:

The Col-0 sequence carrying SNPs, shown in Figure 2 (a), showed identity to three cDNA sequences.

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