Open Access Highly Accessed Research article

Yeast-based assay identifies novel Shh/Gli target genes in vertebrate development

Luis A Milla13, Claudio R Cortés13, Christian Hodar Q23, Maritza G Oñate13, Veronica Cambiazo23, Shawn M Burgess4 and Verónica Palma13*

  • * Corresponding author: Verónica Palma vpalma@uchile.cl

  • † Equal contributors

Author Affiliations

1 Faculty of Sciences, Universidad de Chile, Santiago, Chile

2 Laboratorio de Bioinformática y Expresión Génica, INTA, Universidad de Chile, Santiago, Chile

3 FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

4 National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA

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BMC Genomics 2012, 13:2  doi:10.1186/1471-2164-13-2

Published: 3 January 2012

Additional files

Additional file 1:

Table listing all binding sites found in Shh/Gli yeast assay through our screening strategy. Total mouse GBS positive sequences mapped to specific genes based on shortest distance to the transcriptional start sites.

Format: XLS Size: 88KB Download file

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Additional file 2:

GBS interspecies conservation. in silico analysis of GBS found in the promoter of selected vertebrate genes.

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Additional file 3:

Direct regulation of novel Shh/Gli target genes in the CH310T1/2 murine cell line. CHX 24 hours treatment confirm direct regulation for canonical Shh regulated genes.

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Additional file 4:

Table listing all primers used in this study. Primers designed for control and novel Shh/Gli target gene quantitative PCR assays.

Format: DOCX Size: 65KB Download file

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Additional file 5:

Analysis of in-vivo drug response based on ptc1 expression. Control of zebrafish long-term pharmacological Hedgehog gain and loss of function treatment. ptc1 readout-gene expression was verified by in situ hybridization.

Format: PDF Size: 549KB Download file

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