Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Research article

Yeast-based assay identifies novel Shh/Gli target genes in vertebrate development

Luis A Milla13, Claudio R Cortés13, Christian Hodar Q23, Maritza G Oñate13, Veronica Cambiazo23, Shawn M Burgess4 and Verónica Palma13*

  • * Corresponding author: Verónica Palma vpalma@uchile.cl

  • † Equal contributors

Author Affiliations

1 Faculty of Sciences, Universidad de Chile, Santiago, Chile

2 Laboratorio de Bioinformática y Expresión Génica, INTA, Universidad de Chile, Santiago, Chile

3 FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

4 National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA

For all author emails, please log on.

BMC Genomics 2012, 13:2  doi:10.1186/1471-2164-13-2

Published: 3 January 2012

Abstract

Background

The increasing number of developmental events and molecular mechanisms associated with the Hedgehog (Hh) pathway from Drosophila to vertebrates, suggest that gene regulation is crucial for diverse cellular responses, including target genes not yet described. Although several high-throughput, genome-wide approaches have yielded information at the genomic, transcriptional and proteomic levels, the specificity of Gli binding sites related to direct target gene activation still remain elusive. This study aims to identify novel putative targets of Gli transcription factors through a protein-DNA binding assay using yeast, and validating a subset of targets both in-vitro and in-vivo. Testing in different Hh/Gli gain- and loss-of-function scenarios we here identified known (e.g., ptc1) and novel Hh-regulated genes in zebrafish embryos.

Results

The combined yeast-based screening and MEME/MAST analysis were able to predict Gli transcription factor binding sites, and position mapping of these sequences upstream or in the first intron of promoters served to identify new putative target genes of Gli regulation. These candidates were validated by qPCR in combination with either the pharmacological Hh/Gli antagonist cyc or the agonist pur in Hh-responsive C3H10T1/2 cells. We also used small-hairpin RNAs against Gli proteins to evaluate targets and confirm specific Gli regulation their expression. Taking advantage of mutants that have been identified affecting different components of the Hh/Gli signaling system in the zebrafish model, we further analyzed specific novel candidates. Studying Hh function with pharmacological inhibition or activation complemented these genetic loss-of-function approaches. We provide evidence that in zebrafish embryos, Hh signaling regulates sfrp2, neo1, and c-myc expression in-vivo.

Conclusion

A recently described yeast-based screening allowed us to identify new Hh/Gli target genes, functionally important in different contexts of vertebrate embryonic development.

Keywords:
Hh/Gli targets; zebrafish; purmorphamine; cyclopamine; neogenin 1; c-myc; sfrp2