Table 1

Summary of RNA-seq libraries and flow cells
Lane Flow cell A Lane Flow cell B Lane Flow cell C
1A library 1 (4.5 pM) 1B library 12 (5.5 pM) 1 C library 2 (5.5 pM)
2A library1 (5.0 pM) 2B library 6 (5.5 pM) 2 C library 3 (5.5 pM)
3A library 5 (5.5 pM) 3B library 2 (5.5 pM) 3 C library 6 (5.5 pM)
4A library5 (6.0 pM) 4B library 7 (5.5 pM) 4 C library 7 (5.5 pM)
5A library 10 (4.0 pM) 5B library 9 (5.5 pM) 5 C PhiX
6A PhiX 6B PhiX 6 C library 8 (5.5 pM)
7A library 10 (5.5 pM) 7B library 11 (5.5 pM) 7 C library 11 (5.5 pM)
8A library 10 (6.5 pM) 8B library 4 (5.5 pM) 8 C library 12 (5.5 pM)

Libraries 1–6 = mild negative energy balance animals. Libraries 7–12 = severe negative energy balance animals. Libraries on the first flowcell were run at different concentrations (4.5–6.5 pM) to determine which concentration yielded optimum cluster yields.

McCabe et al.

McCabe et al. BMC Genomics 2012 13:193   doi:10.1186/1471-2164-13-193

Open Data