Additional file 2.
Validation of microarray results. The relative expression of four differentially-expressed genes from the microarray analyses were validated by QRT-PCR. Four genes were selected based on their expression profile between two groups of RILs having the lower (R149 and 29) and the higher (RILs 15 and 64) ascorbic acid contents. Z-score are shown in the microarray data. Mean values ± SE are shown. Genes: Gene3, SGN-U220976; Gene6, SGN-U226214; Gene7, SGN-U220900; Gene12, SGN-U213289. GAPDH (acc. U580213) was used to normalize data.
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Lima-Silva et al. BMC Genomics 2012 13:187 doi:10.1186/1471-2164-13-187