Open Access Highly Accessed Research article

Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit

Viviana Lima-Silva1, Abel Rosado1, Vitor Amorim-Silva2, Antonio Muñoz-Mérida3, Clara Pons4, Aureliano Bombarely5, Oswaldo Trelles3, Rafael Fernández-Muñoz6, Antonio Granell4, Victoriano Valpuesta1 and Miguel Ángel Botella17*

Author Affiliations

1 Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento Biología Molecular y Bioquímica, Universidad de Málaga, 29071, Málaga, Spain

2 BioFIG - Center for Biodiversity, Functional & Integrative Genomics, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, Portugal

3 Computer Architecture Department, University of Malaga, Campus de Teatinos, 29071, Málaga, Spain

4 Instituto de Biología Molecular y Celular de Plantas, Consejo Superior deInvestigaciones Científicas, Universidad Politécnica de Valencia, 46022, Valencia, Spain

5 Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY, 14853, USA

6 Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Est. Exp. La Mayora, Algarrobo-Costa, Málaga, Spain

7 Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Málaga, Spain

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BMC Genomics 2012, 13:187  doi:10.1186/1471-2164-13-187

Published: 14 May 2012

Additional files

Additional file 1:

List of genes differentially expressed between RILs with contrasting content of AsA. Unigene annotations based on the Sol Genomic Networks web site (http://solgenomics.net webcite), and the accession numbers corresponding to Gene bank best hit and the Arabidopsis orthologs identified using Blast2go [35] are included. Genes are grouped according to the clusters obtained after SOTA analysis.

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Additional file 2:

Validation of microarray results. The relative expression of four differentially-expressed genes from the microarray analyses were validated by QRT-PCR. Four genes were selected based on their expression profile between two groups of RILs having the lower (R149 and 29) and the higher (RILs 15 and 64) ascorbic acid contents. Z-score are shown in the microarray data. Mean values ± SE are shown. Genes: Gene3, SGN-U220976; Gene6, SGN-U226214; Gene7, SGN-U220900; Gene12, SGN-U213289. GAPDH (acc. U580213) was used to normalize data.

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