Figure 9.

Comparison of microarray degradation measures (dk and 5′/3′-ratio of the hybridization controls) with the RNA integrity number (RIN, panel a and c) and with the mean length of the transcripts (panel b) obtained in the ratQC experiment. [10] The dk parameter split into two branches for the two sample treatments when plotted as a function of RIN whereas the dk data virtually merge into one branch if plotted as a function of transcript length. Panel d correlates the dk and the 5′/3′- intensity ratio of the control probes in logarithmic scale. The vertical and horizontal orange lines indicate the respective quality thresholds. Good RNA-quality probes are found in direction of the arrows

Fasold and Binder BMC Genomics 2012 13:186   doi:10.1186/1471-2164-13-186
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