Open Access Research article

Lipopolysaccharide priming enhances expression of effectors of immune defence while decreasing expression of pro-inflammatory cytokines in mammary epithelia cells from cows

Juliane Günther1, Wolfram Petzl2, Holm Zerbe2, Hans-Joachim Schuberth3, Dirk Koczan4, Leopold Goetze5 and Hans-Martin Seyfert1*

Author Affiliations

1 Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany

2 Clinic for Ruminants, Ludwig-Maximilians-University Munich, Sonnenstr. 16, D-85764 Oberschleißheim, Germany

3 Institute of Immunology, University of Veterinary Medicine, Bischofsholer Damm 15, D-30173 Hannover, Germany

4 Institute for Immunology, Medical Faculty, University of Rostock, Schillingallee 70, D-18055 Rostock, Germany

5 Pfizer Animal Health, Linkstr. 10, D-10785 Berlin, Germany

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BMC Genomics 2012, 13:17  doi:10.1186/1471-2164-13-17

Published: 12 January 2012

Additional files

Additional file 1:

Table S1: All DEG from comparison Priming (P.) versus Control (C.). A) Short time waiting experiment (40 IPA mapped DEG). B) Long time waiting experiment (13 IPA mapped DEG)

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Additional file 2:

Table S2: All DEG from comparison Induction post Priming (I.p.P.) versus Induction (I.). A) Short time waiting experiment (226 IPA mapped DEG). B) Long time waiting experiment (6 IPA mapped DEG)

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Additional file 3:

Table S3: Comparison of RT-qPCR and microarray measurements of selected candidate genes

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Additional file 4:

Table S4: All RT-qPCR values (relative mRNA copy numbers) contributing to Figure 4.

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Additional file 5:

Table S5: Correlation of relative mRNA concentrations determined in microarray hybridizations or RT-qPCR from three biological pbMEC replica of the four challenge groups (C., P., I., and I.p.P.) of the short and long waiting experiments

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Additional file 6:

Table S6: Sequences of oligonucleotide primers used for real-time PCR quantification

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