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Open Access Highly Accessed Research article

Genome organization and characteristics of soybean microRNAs

Marie Turner1, Oliver Yu2 and Senthil Subramanian1*

Author Affiliations

1 Plant Science Department, South Dakota State University, Brookings, SD, 57007, USA

2 Donald Danforth Plant Science Center, St Louis, MO, 63132, USA

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BMC Genomics 2012, 13:169  doi:10.1186/1471-2164-13-169

Published: 4 May 2012

Additional files

Additional file 1:

Table S1. List of miRNA genes identified and used in this study.

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Additional file 2:

Figure S1. Novel miRNAs identified in this study. Figure S2. Tandem duplication of MIR159 and MIR395 genes. Figure S3. Nucleotide distribution of mature miRNA sequences from different classes of soybean miRNA families. Figure S4. Dissociation curves and amplification efficiency of qPCR assays.

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Additional file 3:

Table S2. Intragenic soybean miRNAs, and miRNAs located less than 1Kb from a protein-coding gene. Table S4. miRNA targets tested negative for miRNA-mediated cleavage as assayed by a modified 5’RLM-RACE assay. Table S5. List of primers used in this study.

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Additional file 4:

Table S3. Target prediction for all available miRNAs in soybean.

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Open Data