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Open Access Highly Accessed Methodology article

GeSICA: Genome segmentation from intra-chromosomal associations

Lin Liu1, Yiqian Zhang1, Jianxing Feng1, Ning Zheng2, Junfeng Yin2 and Yong Zhang1*

Author affiliations

1 Department of Bioinformatics, School of Life Science and Technology, Tongji University, Shanghai, 200092, China

2 Department of Mathematics, Tongji University, Shanghai, 200092, China

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Citation and License

BMC Genomics 2012, 13:164  doi:10.1186/1471-2164-13-164

Published: 4 May 2012

Abstract

Background

Various aspects of genome organization have been explored based on data from distinct technologies, including histone modification ChIP-Seq, 3C, and its derivatives. Recently developed Hi-C techniques enable the genome wide mapping of DNA interactomes, thereby providing the opportunity to study genome organization in detail, but these methods also pose challenges in methodology development.

Results

We developed Genome Segmentation from Intra Chromosomal Associations, or GeSICA, to explore genome organization and applied the method to Hi-C data in human GM06990 and K562 cells. GeSICA calculates a simple logged ratio to efficiently segment the human genome into regions with two distinct states that correspond to rich and poor functional element states. Inside the rich regions, Markov Clustering was subsequently applied to segregate the regions into more detailed clusters. The binding sites of the insulator, cohesion, and transcription complexes are enriched in the boundaries between neighboring clusters, indicating that inferred clusters may have fine organizational features.

Conclusions

Our study presents a novel analysis method, known as GeSICA, which gives insight into genome organization based on Hi-C data. GeSICA is open source and freely available at: http://web.tongji.edu.cn/~zhanglab/GeSICA/ webcite