Research article
Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples
1 Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Athenry, Galway, Ireland
2 Grange, Dunsany, Meath, Ireland
3 Moorepark, Fermoy, Cork, Ireland
4 Animal Genomics Laboratory, UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
5 Department of Animal Sciences, University of Missouri, Columbia, USA
6 UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
BMC Genomics 2012, 13:16 doi:10.1186/1471-2164-13-16
Published: 11 January 2012Additional files
Additional file 1:
Excel file containing the four additional tables. Additional Table 1 - details of the genes including sequence co ordinates selected for target enrichment and sequencing. Additional Table 2 - details of the SNPs and indels identified across the 83 candidate genes in both the low and high calving interval DNA pools. Additional Table 3 - details of all SNPs reported on dbSNPv130 within the 1.5 Mb DNA sequences targeted for sequencing. Additional Table 4 - 24 undetected SNPs validated as segregating in the 150 Holstein-Friesian sires using Sequenom® MassARRAY genotyping.
Format: XLSX Size: 878KB Download file
