Table 2

qPCR validation of results and microarray data obtained from stage 17 embryos

microarray

qPCR


ManfmzΔ96vs w

ManfmzΔ96vs w

69B>Manf133 vs w


#

Gene Name

log FC

P.Value

log2

T-test

log2

T-test

gene ID

Description


1

CG10420

1.98

0.0001

5.15

9.7E-07

-3.29

1.8E-05

CG10420

Nucleotide exchange factor SIL1 precursor


2

CG14879

-4.03

7.3E-11

-3.46

0.0004

CG14879

Concanavalin A like lectin homology


3

CG5810

-1.92

1.8E-06

-3.78

1.5E-07

CG5810

leucine rich repeat


4

Ddc

1.62

4.6E-05

2.69

0.0003

-4.99

1.6E-05

CG10697

DOPA decarboxylase


5

DnaJ-H

1.10

4.1E-05

2.56

2.3E-05

CG9828

DnaJ homolog


6

Hrs

1.56

0.0001

1.38

0.05

CG2903

Hepatocyte growth factor-regulated tyrosine kinase substrate


7

Hsp83

2.46

9.7E-05

4.57

3.2E-06

CG1242

Heat shock protein 83, HSP90 homolog


8

InR

1.21

0.0007

1.87

3.4E-05

CG18402

Insulin-like receptor precursor


9

Pak3

-1.96

0.0003

-1.14

0.0003

CG14895

Pak, serine threonine kinase


10

Pi3K92E

1.11

0.0003

2.34

0.03

CG4141

phosphatidylinositol-4-phosphate 3-kinase


11

pipe

-1.53

4.2E-05

-0.25

0.0001

CG9614

heparan sulfate 2-O-sulfotransferase


12

pale

2.83

3.4E-07

4.83

1.0E-06

CG10118

TH; Tyrosine 3-hydroxylase


13

punch

1.97

4.3E-06

8.41

0.0001

0.69

0.02

CG9441

punch, GTP cyclohydrolase I


14

Rala

1.48

1.7E-07

0.63

2.1E-05

CG2849

Ras-related protein


15

sulfateless

4.21

1.8E-09

1.62

4.3E-05

CG8339

heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase


16

ROP

3.33

4.2E-10

0.82

4.3E-05

CG15811

Ras-opposite


17

ubisnap

-0.43

4.4E-05

-1.18

0.001

CG11173

ubisnap, SNAP29 homolog


18

DmManf

-1.37

0.0006

-5.39

6.3E-06

1.82

1.8E-06

CG7013

Manf, known previously as Arp-like


Only statistically significant results (P < 0.05) are presented. Results of qPCR were made comparable to microarray fold change (FC) by calculating the log2 from the relative fold changes. T-test means calculated P-value associated with Student's t-Test.

Palgi et al. BMC Genomics 2012 13:134   doi:10.1186/1471-2164-13-134

Open Data