Open Access Highly Accessed Research article

Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes

Mari Palgi12*, Dario Greco23, Riitta Lindström12, Petri Auvinen2 and Tapio I Heino12*

Author Affiliations

1 Department of Biosciences, University of Helsinki, PO Box 56, Viikinkaari 5, Helsinki 00014, Finland

2 Institute of Biotechnology, University of Helsinki, PO Box 56, Viikinkaari 9, Helsinki 00014, Finland

3 Department of Bioscience and Nutrition, Karolinska Institutet, Stockholm, Sweden

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BMC Genomics 2012, 13:134  doi:10.1186/1471-2164-13-134

Published: 11 April 2012

Additional files

Additional file 1:

Table of all obtained qPCR results. The Excel file contains two separate sheets, one for the data obtained from stage 17 embryos and the other for the data obtained from 29-50 hr AEL larvae.

Format: XLSX Size: 21KB Download file

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Additional file 2:

DAVID functional annotation clustering analysis of downregulated genes in embryonic ManfmzΔ96 mutants. An Excel file; 1191 genes were downregulated and grouped into 105 functional clusters.

Format: XLSX Size: 144KB Download file

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Additional file 3:

DAVID functional annotation clustering analysis of upregulated genes in embryonic ManfmzΔ96 mutants. An Excel file; 1243 genes were upregulated and grouped into 230 functional clusters.

Format: XLSX Size: 260KB Download file

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Additional file 4:

DAVID functional annotation clustering analysis of downregulated genes in larval ManfΔ96 mutants. An Excel file; 690 genes were downregulated and grouped into 140 functional clusters.

Format: XLSX Size: 167KB Download file

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Additional file 5:

DAVID functional annotation clustering analysis of upregulated genes in larval ManfΔ96 mutants. An Excel file; 682 genes were upregulated and grouped into 122 functional clusters.

Format: XLSX Size: 144KB Download file

Open Data

Additional file 6:

DAVID functional annotation clustering analysis of common downregulated genes in ManfmzΔ96 and ManfΔ96 mutants. An Excel file; 208 genes were downregulated in both Manf mutants and grouped into 47 functional clusters.

Format: XLSX Size: 57KB Download file

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Additional file 7:

DAVID functional annotation clustering analysis of common upregulated genes in ManfmzΔ96 and ManfΔ96 mutants. An Excel file; 229 genes were upregulated in both Manf mutants and grouped into 45 functional clusters.

Format: XLSX Size: 75KB Download file

Open Data

Additional file 8:

Exocytosis and SNARE complex is altered in Manf mutants. A pdf file; an online coloured KEGG pathway showing altered gene expression in either red (upregulation), blue (downregulation), or in purple (altered gene expression) boxes. The unaltered known Drosophila homologues to identified components from other organisms are presented in green-filled boxes. The complete list of altered genes is summarised in Table 8.

Format: PDF Size: 229KB Download file

This file can be viewed with: Adobe Acrobat Reader

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Additional file 9:

Lysosomal degradation is altered in Manf mutants. A pdf file; an online coloured KEGG pathway showing altered gene expression in either red (upregulation), blue (downregulation), or in purple (altered gene expression) boxes. The unaltered known Drosophila homologues to identified components from other organisms are presented in green-filled boxes. The complete list of altered genes is summarised in Table 10.

Format: PDF Size: 417KB Download file

This file can be viewed with: Adobe Acrobat Reader

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Additional file 10:

DAVID functional annotation clustering analysis of upregulated genes in ManfmzΔ96 mutant paternal rescue. An Excel file; 98 genes were upregulated when maternal lack of Manf gene products was rescued paternally and grouped into 18 functional clusters.

Format: XLSX Size: 29KB Download file

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Additional file 11:

DAVID functional annotation clustering analysis of downregulated genes in ManfmzΔ96 mutant paternal rescue. An Excel file; only 34 genes were downregulated when maternal lack of Manf gene products was rescued paternally and grouped into 4 functional clusters.

Format: XLSX Size: 15KB Download file

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Additional file 12:

DAVID functional annotation clustering analysis of upregulated genes in Manf ectopic overexpression by 69B-GAL4. An Excel file; 614 genes were upregulated and grouped into 102 functional clusters.

Format: XLSX Size: 125KB Download file

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Additional file 13:

DAVID functional annotation clustering analysis of downregulated genes in Manf ectopic overexpression by 69B-GAL4. An Excel file; 340 genes were downregulated and grouped into 78 functional clusters.

Format: XLSX Size: 157KB Download file

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Additional file 14:

DAVID functional annotation clustering analysis of common upregulated genes in ManfmzΔ96 paternally rescued embryos and in Manf ectopically overexpressing larvae. An Excel file; 57 genes were upregulated in both Manf mutants and grouped into 9 functional clusters, 14 GO terms remained unclustered.

Format: XLSX Size: 19KB Download file

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Additional file 15:

Complete list and DAVID functional annotation clustering analysis of genes downregulated in ManfΔ96 mutants and upregulated in Manf ectopic overexpression and vice versa. An Excel file; 46 genes were downregulated in larval ManfΔ96 mutants and upregulated in Manf ectopic overexpression, vice versa 16 genes were upregulated in ManfΔ96 mutants and downregulated in Manf ectopic overexpression. In the first sheet complete list of genes together with fold change logarithmic (FClog) results is presented. In the second sheet results of DAVID functional annotation clustering analysis with 9 clusters is shown.

Format: XLSX Size: 31KB Download file

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Additional file 16:

List of all designed primers used for qPCR.

Format: PDF Size: 5KB Download file

This file can be viewed with: Adobe Acrobat Reader

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