PCR verifications of the unique presence or absence of TCS genes. The unique absence of TCS-3 and TCS-9 in S. sorbrinus DSM20742 are demonstrated by showing the absences of the HK of its TCS-3 and the HK and RR of its TCS-9, in contrast to the presence of the corresponding genes in other strains, as exemplified in S. mutans UA159 and S. ratti DSM20564. The absence of TCS-13 and the presence of TCS-18 in S. ratti DSM20564 are presented in contrast to S. mutans UA159 and S. sorbrinus DSM20742. The unique presence of TCS-15 in S. mutans NCTC11060 was verified using two forward primers. The sequences of the primers used for PCR are provided in the Materials and Methods section.
Song et al. BMC Genomics 2012 13:128 doi:10.1186/1471-2164-13-128