Growth characteristics of wild type, Δvvd, Δwc-1 and Δwc-2 mutants. (A) extracellular protein production. The wild type strain secreted 42.7 ± 5.1 mg/L of protein after 28 hours and 51.7 ± 2.4 mg/L of protein after 40 hours of cultivation on cellulose. (B) NaOH soluble protein of mycelia reflecting biomass accumulation of WT and mutant strains after 28 or 40 hours of growth on cellulose, relative to WT at the respective time point. (C) Specific Azo- CMC cellulase activity (cellulase activity per biomass) after 28 or 40 hours of growth on cellulose relative to wild type at the respective time point. Error bars show standard deviation of at least two biological replicates.
Schmoll et al. BMC Genomics 2012 13:127 doi:10.1186/1471-2164-13-127