Alternative splicing validations for two genes using PCR with cDNA as the template. Lanes A and B correspond to the two fragment sizes for the gene AP2B1 (134 and 92 bp) while G and H represent the two transcripts for ZDHHC16 (97 and 118 bp). Lanes C and I are positive controls (GBG5, 115 bp) while D, E, F, J, K, and L are negative controls.
Driver et al. BMC Genomics 2012 13:118 doi:10.1186/1471-2164-13-118