B-specific sequences of D. albomicans. (A) Validation of the identified B-linked probe with Southern Blotting. Up panel: Genomic DNAs digested with HaeII. Lanes 3-5,10 show hybridization results with genomic DNA from D. albomicans strains without B's. Lane 11 is the negative control lane using genomic DNA of D. melanogaster. Other lanes show hybridizations with DNA from D. albomicans strains containing B's. Arrows indicate differential bands between B and no-B strains. Down panel: Genomic DNAs digested with MspI. Lanes 3-6 show D. albomicans strains without B's. Lane 8 is the negative control and other lanes are D. albomicans strains with B's. (B) PCR assays using B-chromosome derived primers. Lane 2-5 show PCR products obtained from D. albomicans strains without B's, and lanes 6-9 are from strains with B's. Lane 1 is the DL2000 marker.
Zhou et al. BMC Genomics 2012 13:109 doi:10.1186/1471-2164-13-109