Table 1 |
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|
Statistics of small RNA sequences from Chlamydomonas reinhardtii + S and -S libraries |
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|
Sequences generated |
% |
|
|
|
||
|
+S library |
||
|
Total reads |
11284767 |
|
|
High quality |
10762852 |
100% |
|
Adaptor3 null |
3304 |
0.03% |
|
Insert null |
2815 |
0.03% |
|
Adaptor5 contaminants |
63608 |
0.59% |
|
Smaller than 18 nt |
663116 |
6.16% |
|
PolyA |
17 |
0.00% |
|
Clean reads |
10029992 |
93.19% |
|
-S library |
||
|
Total reads |
11080539 |
|
|
High quality |
10572756 |
100% |
|
Adaptor3_null |
3499 |
0.03% |
|
Insert null |
3575 |
0.03% |
|
Adaptor5 contaminants |
58280 |
0.55% |
|
Smaller than 18 nt |
588466 |
5.57% |
|
PolyA |
6 |
0.00% |
|
Clean reads |
9918931 |
93.82% |
|
|
||
|
Total reads: total sequenced reads; High quality: number of high quality reads with no N, no more than 4 bases whose quality score is lower than 10 and no more than 6 bases whose quality is lower than 13; Adaptor3 null: number of reads with no 3' adaptor; Insert null: number of reads with no insertion; Adaptor5 contaminants: number of 5' contaminants; Smaller than 18 nt: number of reads less than 18 nt. Generally, small RNA tags are between 18 ~ 30 nt long. So too short tags should be removed from data for future analysis; polyA: number of reads wih polyA; Clean reads: number of clean reads after adaptors and contaminants are removed which are used in the following analysis. |
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|
Shu and Hu BMC Genomics 2012 13:108 doi:10.1186/1471-2164-13-108 |
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