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Open Access Highly Accessed Research article

Genome-wide dynamic transcriptional profiling in clostridium beijerinckii NCIMB 8052 using single-nucleotide resolution RNA-Seq

Yi Wang12, Xiangzhen Li34, Yuejian Mao3 and Hans P Blaschek256*

Author Affiliations

1 Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

2 Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

3 Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

4 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu Sichuan 610041, China

5 Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

6 Center for Advanced Bioenergy Research (CABER), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

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BMC Genomics 2012, 13:102  doi:10.1186/1471-2164-13-102

Published: 20 March 2012

Additional files

Additional file 1:

The genes with Z-score > 1.7 in each sample.

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Additional file 2:

The top 100 highest expressed genes in samples B and D.

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Additional file 3:

The expression of the putative alcohol dehydrogenase genes in C. beijerinckii 8052.

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Additional file 4:

The putative signal transduction genes in SOM clusters C8-C10 in C. beijerinckii 8052.

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Additional file 5:

The qRT-PCR verification of RNA-Seq quantification results for selected genes. The fold change of each gene's expression along time course measured by either qRT- PCR (in black) or RNA-Seq (in red) was compared to that of the same gene at first time point (time point A in Figure 1A). (A) Cbei_4852, pfk. (B) Cbei_1903, fba. (C) Cbei_4851, pyk. (D) Cbei_0203, ptb. (E) Cbei_0204, buk. (F) Cbei_3833, ctfA. (G) Cbei_3834, ctfB. (H) Cbei_0411, thlA. (I) Cbei_0325, hbd.

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Additional file 6:

RNA-Seq data analysis.

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Additional file 7:

Genes and primer sequences for qRT-PCR test.

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