Table 1

Average GC content

Sample

Library

PCR-free

Kapa HiFi

Kapa2G

AccuPrime

Platinum

RPA

T7

Phusion


3D7

Av. %GC content

19.50

20.35

21.44

21.93

21.47

20.75

22.63

23.80

PK0076

Av. %GC content

21.92

19.79

21.07

21.37

21.91

19.66

20.95

22.87


Calculated values of average GC content corresponding to each library preparation. Deviation from the 19.4% (value of the unamplified genome) indicates the effect of amplification bias. See Figure 3 A & B for a global graphical presentation of the GC content distribution. 3D7, P. falciparum strain 3D7; PK0076, P. falciparum clinical isolate.

Oyola et al. BMC Genomics 2012 13:1   doi:10.1186/1471-2164-13-1

Open Data