Figure 5.

Detecting novel organisms Because MetaPhyler uses different classification thresholds for different phylogenetic levels, it can avoid assigning an organism to a lower-level taxonomic group if the evidence does not support this assignment. The presence of novel organisms leads to a detectable discrepancy between the number of sequences assigned to a lower taxonomic level, and the number of sequences assigned to a higher (less specific) taxonomic level.

Liu et al. BMC Genomics 2011 12(Suppl 2):S4   doi:10.1186/1471-2164-12-S2-S4