Estimating taxonomic profiles using 16S rRNA targeted sequencing or metagenome shotgun sequencing. Figure1a shows that the taxonomic profile estimated from 16S rRNA targeted sequencing is biased because of copy number variation. Figure 1b shows that classification of whole-metagenome shotgun sequences may produce biased estimation because of the variations in genome size.
Liu et al. BMC Genomics 2011 12(Suppl 2):S4 doi:10.1186/1471-2164-12-S2-S4