Additional file 1.
β-lactamase assay on TAT library. Panel A shows 45-48 TAT clones for each filtering concentration in the 96 well format nitrocefin (NC) assay. Clones correctly expressing a functional β-lactamase fusion in the supernatant turn from yellow to red. Controls are shown in black circles. In panel B, absorbance measurements performed at 2 h, 6 h, and 16 h (saturation point) are shown as average measurement of triplicate plates. Data were normalized on the positive control signal (shown as black bar with 100% signal) and reported as percentage value.
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D'Angelo et al. BMC Genomics 2011 12(Suppl 1):S5 doi:10.1186/1471-2164-12-S1-S5