Subcellular localization prediction of JGI v4.0 enzymes. Following enzyme classification assignments to JGI v4.0 translated ORFs, subcellular localization of the proteins were predicted by WoLF PSORT  as plant (A) or animal (B) proteins. Based on the obtained probability values, each protein was assigned a compartment when 50% or higher percentage of the nearest neighbors for the protein belonged to a given compartment. When the 50% threshold is not reached, the protein, or its encoding ORF are assigned to “other” category to designate multiple compartments or ambiguous predictions. In (C), the predictions made as animal and plant were consolidated into a single set by increasing the threshold to 85%, then reporting the predicted assignment with the higher value. Abbreviations are: Chlo: chloroplast, Cyto: cytosol, Cysk: cytoskeleton, E.R.: endoplasmic reticulum, Extr: extracellular, Mito: mitochondrion, Nuc: nucleus, Pero: peroxisome, Plas: plasma membrane, Vacu: vacuolar membrane.
Ghamsari et al. BMC Genomics 2011 12(Suppl 1):S4 doi:10.1186/1471-2164-12-S1-S4