Table 4

Differentially expressed proteins in ΔsRNA-Xoo1 identified by MS

Spot ID

protein name

functions

NCBI acc.no.

mascot score

Sequence coverage

theoretical

MW(Da)/pI

Ratio ± SD


Down-regulated protein spots in sRNA-Xoo1 mutant

B1

putative ABC transporter ATP-binding protein

transport

PXO_04409

448

56%

61740/5.37

11.57 ± 4.83

B2

glyceraldehyde-3-phosphate dehydrogenase

metabolism

PXO_02308

529

69%

36134/6.35

4.75 ± 0.57**

B3

argininosuccinate synthase

amino acid biosynthesis

PXO_00352

769

67%

45217/5.66

3.25 ± 0.56

B4

tryptophan repressor binding protein

amino acid metabolism

PXO_03044

438

52%

20167/6.05

2.83 ± 0.18**

B5

3-isopropylmalate isomerase large subunit

amino acid biosynthesis

PXO_02613

314

42%

51984/5.68

1.86 ± 0.21**

B6

N-acetylornithine carbamoyltransferase

amino acid biosynthesis

PXO_00353

314

65%

37582/6.07

1.7 ± 0.19**

B7

superoxide dismutase

oxidation reduction

PXO_00389

343

68%

22703/5.47

1.42 ± 0.03**

B8

septum site-determining protein MinD

replication

PXO_04464

435

46%

28837/5.21

1.57 ± 0.19**

B9

N-ethylammeline chlorohydrolase

metabolism

PXO_00380

263

62%

48943/5.19

ND

B10

2-isopropylmalate synthase

amino acid biosynthesis

PXO_02609

694

71%

53421/5.54

1.54 ± 0.15**

B11

translation elongation factor G

transcription and translation

PXO_04525

642

58%

75924/5.09

4.82 ± 0.42**

B12

dihydrolipoamide dehydrogenase

amino acid metabolism

PXO_01196

582

67%

50445/5.98

2.29 ± 0.54

B13

N-acylglucosamine 2-epimerase

metabolism

PXO_01231

811

77%

46884/5.27

2.23 ± 0.34**

B14

ATP synthase subunit alpha

transport

PXO_03111

777

48%

55391/5.38

2.23 ± 0.5

B15

phosphoenolpyruvate synthase

Pyruvate metabolism

PXO_00922

622

48%

86689/5.16

6.36 ± 1.69

B16

Polyribonucleotide nucleotidyltransferase

metabolism

PXO_01307

729

55%

75502/5.47

5.39 ± 0.93

Up-regulated protein spots in ΔsRNA-Xoo1 mutant

B17

pyrroline-5-carboxylate reductase

amino acid metabolism

PXO_01991

301

51%

29364/4.93

1.48 ± 0.07**

B18

peptide deformylase

protein synthesis

PXO_04055

379

55%

17129/5.29

1.45 ± 0.02**

B19

outer membrane protein

transport

PXO_03097

343

32%

23707/5.27

1.43 ± 0.04**

B20

Chaperone protein dnaJ

DNA replication

PXO_01186

702

64%

41079/6.21

1.52 ± 0.14**

B21

hydrolase, carbon-nitrogen family

metabolism

PXO_06060

526

68%

30229/5.89

1.57 ± 0.12**

B22

hypothetical protein

hypothetical protein

PXO_01766

635

75%

16502/6.3

1.41 ± 0.01


ND: not detectable in the 2-DE maps of wild-type or ΔsRNA-Xoo1 mutant. SD: Standard Deviation. Statistical significance was determined using Student's two-tailed t test for unpaired means P < 0.05, P < 0.01**.

Liang et al. BMC Genomics 2011 12:87   doi:10.1186/1471-2164-12-87

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