Additional file 6.
RT-PCR confirmation of the transcriptional unit of the hfq gene (pdf). (A) The position and direction of ORFs were presented by arrows, and the corresponding names for each ORFs were also showed. The locations of primers used in the following PCR were presented by arrows. (B) RNAs prepared from wild-type cells cultured in rich (R) and minimum (M) medium were used for the reverse transcription using random primers to synthesis cDNA separately. DNA, positive control; +, with reverse transcriptase; -, without reverse transcriptase (a negative control to show no contamination of genomic DNA in the RNA sample).
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Liang et al. BMC Genomics 2011 12:87 doi:10.1186/1471-2164-12-87