Open Access Highly Accessed Research article

Potential efficacy of mitochondrial genes for animal DNA barcoding: a case study using eutherian mammals

Arong Luo12, Aibing Zhang3, Simon YW Ho4, Weijun Xu5, Yanzhou Zhang1, Weifeng Shi6, Stephen L Cameron7* and Chaodong Zhu1*

Author Affiliations

1 Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China

2 Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China

3 College of Life Sciences, Capital Normal University, Beijing 100048, PR China

4 School of Biological Sciences, University of Sydney, Sydney NSW 2006, Australia

5 Zhongbei College, Nanjing Normal University, Nanjing 210046, PR China

6 UCD Conway Institute of Biomolecular and Biomedical Sciences, University College Dublin, Dublin 4, Ireland

7 Australian National Insect Collection, CSIRO Entomology, Canberra ACT 2601, Australia

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BMC Genomics 2011, 12:84  doi:10.1186/1471-2164-12-84

Published: 28 January 2011

Additional files

Additional file 1:

Figure of K2P distances versus intra- and interspecific comparisons from 12 profiles. The x-axis represents K2P distance values (%) and the y-axis represents the number of comparisons. The number of comparisons indicates either the number of species compared (intraspecific comparisons, blue) or the number of genera compared (interspecific comparisons, red).

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Additional file 2:

Figure of nucleotide divergence of 10 species pairs of the genus Ursus. Interspecific distances of 10 species pairs (1, U. americanus; 2, U. arctos; 3, U. maritumus; 4, U. spelaeus; 5, U. thibetanus) from the sliding-window analyses are shown. The x-axis represents nucleotide midpoints of the 600 bp window. The range of each gene is annotated with a pair of ticks.

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Additional file 3:

Figure of grouping results from PCOORD for 12 gene profiles. From A to L, the figures show the grouping based on nucleotide sequences; the others are based on amino acid sequences. Symbols indicate: Afrotheria (red circle); Euarchontoglires (grey down-pointing triangle); Laurasiatheria (green up-pointing triangle); and Xenarthra (blue square).

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Additional file 4:

Figure of %Ti values against pairwise K2P distances for 12 gene profiles. The x-axis represents the K2P distance values, while the y-axis represents %Ti values.

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Additional file 5:

Table of information of the genome and gene sequences. Taxon information, accession number and other information are shown for each mitochondrial genome sequence. √denotes the presence of the gene sequence in the aligned gene profile, while * denotes the absence of the sequence in the aligned gene profile. Species names given in blue font indicate extinct species.

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Additional file 6:

Figure of relationship between intraspecific distances and sample sizes. The y-axis represents average intraspecific distances less than 3% of species from the genome profile, while the x-axis represents sample sizes for these species on a log scale.

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