Open Access Highly Accessed Research article

RNA-seq analyses of blood-induced changes in gene expression in the mosquito vector species, Aedes aegypti

Mariangela Bonizzoni12, W Augustine Dunn23, Corey L Campbell4, Ken E Olson5, Michelle T Dimon67, Osvaldo Marinotti2 and Anthony A James28*

  • * Corresponding author: Anthony A James aajames@uci.edu

  • † Equal contributors

Author Affiliations

1 Program in Public Health, University of California, Irvine, California, USA

2 Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA

3 Institute for Genomics and Bioinformatics, University of California, Irvine, California, USA

4 Department of Biochemistry and Molecular Biology, Colorado State University, Ft Collins, Colorado, USA

5 Department of Microbiology, Immunology and Pathology, Colorado State University Fort Collins, Colorado, USA

6 Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA

7 Biological and Medical Informatics Program, University of California San Francisco, San Francisco, California, USA

8 Department of Microbiology and Molecular Genetics, University of California, California, Irvine, USA

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BMC Genomics 2011, 12:82  doi:10.1186/1471-2164-12-82

Published: 28 January 2011

Additional files

Additional file 1:

Comparison of normalized transcript abundance between replicate libraries with respective Pearson correlations. (B) Blood-fed. (S) Sugar-fed. Axes values are in reads transcript-1 library-1. Ba: blood-fed replicate library A. Bb: blood-fed replicate library B. Sa: sugar-fed replicate library A. Sb: sugar-fed replicate library B. The Pearson statistics and equation for the best-fit line are shown in the inset.

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Additional file 2:

List of all the transcripts identified from RNA-seq libraries from blood- and sugar-fed Aedes aegypti females. The number of reads per transcript and the fold-changes in gene expression between blood- and sugar-fed samples also are included. Sheets 2 and 3 list the transcripts found at significant levels only in blood- and sugar-fed mosquitoes, respectively. In sheets 2 and 3, a column with the transcript description as derived from Ensembl Metazoa [60] and three columns with values corresponding to "function parent", "best match to SWISSP database" and "best match to PFAM database" as derived from AegyXcel [27] are included.

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Additional file 3:

List of the transcripts related to pathogen interaction or with putative defense mechanisms, as identified by a comparative genomic analyses in silico [27,37,38,40-47,51,52]. Sheet 2 and 3 list the Anti-Pathogen (AP) transcripts with increased and decreased accumulation in blood fed mosquitoes, respectively.

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Additional File 4:

Motif map of putative CREs discovered by SCOPE using transcripts detected significantly only in blood fed female Ae. aegypti. Locations of representative SCOPE-derived CRE motifs in the 2000 bp upstream of the annotated translational start site in the 40 transcripts detected significantly only in B. Transcript names on the left are ordered from most (top) to least (bottom) abundant.

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Additional file 5:

List of primers used for real-time RT-PCR validation of RNA-seq based data.

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