CNE 3299 harbours essential conserved Pbx-Hox and Meis binding-sites. A, multiple sequence alignment of a region of CNE 3299 from human, zebrafish and lamprey highlighting conserved Pbx-Hox (blue box) and Meis (green box) binding-site motifs. The specific sites mutated in elements sub1 and sub2 are indicated below the alignment. B-C, compared to wild-type 3299 expression (B), mutating the first Pbx-Hox and Meis motif cluster (sub1) results in the loss of reporter expression in the neural crest (arrow) and broader expression in the hindbrain (arrowhead) (C). nc: neural crest.
Parker et al. BMC Genomics 2011 12:637 doi:10.1186/1471-2164-12-637