Screen to isolate L. kluyveri ARSs. (A) L. kluyveri genomic DNA was fragmented with MboI and ligated into the pIL07 vector. The resultant libraries were transformed into L. kluyveri strain FM628 and ARS plasmids were isolated from resulting colonies. (B) Representative colony sizes of plasmids showing ARS activity or the lack thereof.
Liachko et al. BMC Genomics 2011 12:633 doi:10.1186/1471-2164-12-633